Uranyless Ethanol
Uranyless in 30% Ethanol
Ready to Use
UranyLess in 30% ethanol is a reagent specifically developed for tissues that are difficult to contrast, such as very dense, fibrous, or lipid-rich tissues (e.g., cornea). The alcohol acts as a vector, allowing UranyLess to penetrate deep into these tissues and improve contrast.
It serves as an alternative to traditional uranyl acetate for biological sample preparation, providing high-resolution imaging without the use of hazardous materials.
Its ethanol-based formulation enhances contrast penetration while preserving the integrity of lipid structures.
Key Features:
- Prepared in a 30% ethanol solution in water.
- Ethanol enhances contrast penetration, particularly in collagen-rich tissues.
- The 30% ethanol concentration provides an optimal balance for deep tissue penetration while preserving lipid structures.
Sample Preparation Protocol with UranyLess in 30% Ethanol
1. Contrast the Sample:
- Apply UranyLess in ethanol to the sample for 1 to 2 minutes.
2. Wash the Sample:
- Rinse the sample with a drop of 30% alcohol for 1 minute.
3. (Optional) Contrast Enhancement:
- To further enhance contrast, apply lead citrate (#DM22410) for 1 minute.
Transverse and longitudinal sections of corneal fiber bundles
copyright Dr. Chantal Cazveille-INM Montpellier, France
Compare Them !
Aqueous | Acetone | Ethanol | |||
---|---|---|---|---|---|
Volume | 30ml | 200ml | 100ml | 30ml | 200ml |
Reference | 11000 | 11000-200 | 11000C-100 | 11000E-30 | 11000E-200 |
Expiration | 12 Months | 12 Months | 12 Months | 12 Months | 12 Months |
Use | Contrast agent for ultrathin sections or negative staining | Block staining for AFS | Staining of dense and lipid-rich tissues | ||
Cryofixation | |||||
Technique | Manual Staining | Automatic staining for EM stain Leica or RMC grids stainer | Manual Staining | Manual Staining | Automatic staining for EM stain Leica or RMC grids stainer |
Container | Airless bottle, no contact with air | HDPE bottle | Glass bottle | Airless bottle, no contact with air | HDPE bottle |
SEE ALSO
Navigation
Contact
22b route de Saint Ybars 31190
Mauressac France
+(33) 5 61 73 60 14
info@deltamicroscopies.com