Negative Staining Protocol

Negative staining is a very useful technique in electron microscopy that allows for the characterization of the morphology of isolated particles such as bacteria, viruses, proteins, nanoparticles, liposomes, exosomes, etc.

Staining Protocol:

1. On a piece of Parafilm or any other hydrophobic surface, place a drop of approximately 10 µl of your solution and a drop of UranyLess.
2. Using fine tweezers, place a grid coated with a formvar-carbon film on the drop of your sample. Leave in contact for about 1 minute.
3. Drain the grid using filter paper.
4. Contrast the grid by placing it in contact with the drop of UranyLess for 1 minute.
5. Drain, dry for 5 minutes, then observe under the microscope.