STAIN77 – EMstain solution
Stain 77
Stain 77 is an extremely low-toxicity contrast agent composed of lithium and tungsten salts (refer to MSDS). It effectively enhances both positive and negative contrast. The stain is light-insensitive and can be stored long-term at room temperature (RT). If crystallization occurs over time, the product’s contrast-enhancing properties can be restored through filtration or centrifugation
Application
Stain 77 serves as an effective alternative to uranyl acetate for section contrast and negative staining, with a pH close to 7, ensuring user safety. After enhancement with lead citrate, contrast results match or even surpass those achieved with uranyl acetate.
Stain77 Ultra-Thin Section Contrast Protocol:
- Application: Apply a drop of Stain77 to the ultra-thin section for 1 to 2 minutes.
- Drying: Allow the section to dry.
- Rinsing: Briefly rinse with distilled water.
- Drying: Allow the section to dry again.
- Counterstaining: Apply lead citrate for 1 minute.
- Rinsing and Drying: Rinse briefly with distilled water and then dry the section.
- Observation: Examine the section using a Transmission Electron Microscope (TEM).
Please note: Avoid pre-storing rinse water in the refrigerator, as cold water may absorb CO2, potentially contaminating your sections.
This protocol enhances the contrast of ultra-thin sections (positive contrast) and is also suitable for negative staining. However, negative staining with Stain77 does not require counterstaining with lead citrate.
Tips
It’s available in two variants and two volumes (30 ml and 200 ml):
- A 30 ml Airless bottle with a drop-by-drop dispenser for manual staining.
- A 200 ml option for use with the Leica EM AC20 or RMC automated contrast systems (briefly rinse with distilled water for 2×5 minutes).
Stain77 can be stored in the laboratory at room temperature and is not affected by light exposure.
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Mauressac France
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info@deltamicroscopies.com