Animal Tissue

MOUSE
Neuromuscular​
Intestine
Kidney​
Andrenal Gland​
Ovarian

MOUSE AND SAHARA GERBIL LIVER

Sample preparation according to the following protocol: :

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.
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Hepatocyte – perinuclear region
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Overview of a Sahara gerbil hepatocyte.

Neuromuscular

Muscle – Mouse nerve

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – 1 minute UranyLess contrast – 1 minute Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

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Intestine

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

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Kidney

Mouse Kidney

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – 1 minute UranyLess contrast – 1 minute Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

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Mouse kidney
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Basal invaginations – hemi- desmosomes – basal blade: increase the exchange surface – Kidney
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Glomerular Zone – podocytes – stalks – Kidney.
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Podocytes – stalks – Kidney.

Andrenal Gland

Adrenal gland Sahara gerbil

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

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Glomerular Zone – podocytes – stalks – Kidney.
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Adrenal gland Sahara gerbil

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

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Mouse kidney
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Basal invaginations – hemi- desmosomes – basal blade: increase the exchange surface – Kidney

Ovarian - Ovarian Follicle - Uranyless

Mouse ovarian follicle

We have tested UranyLess on different accessories cells constituting the mouse ovarian follicle.

Sample preparation according to the following protocol:

  • Classic PFA- Glutaraldehyde, Osmium, Epon fixing.
  • Ultrafine section – UranyLess contrast – Lead Citrate.

Photos taken with a Hitachi HT7700 Transmission Electron Microscope by Nacer BENMERADI (R&D – Delta Microscopies – France).

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Internal theca of mouse ovarian follicle.
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Mitochondria fingers typical glove steroid synthesis in cells (Internal theca of mouse ovarian follicle).
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External theca cell of a mouse ovarian follicle.
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Follicular cell of the mouse ovarian follicle Corona Radiata.

Cardiac muscle - Uranyless

Mouse cardiac muscle

Preparation of the sample using following protocol:

  • classic Fixing Glutaraldehyde, Osmium, Epon Ultrafine
  • Cups, Double Uranyless Contrast and Lead Citrate
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uranyless logo

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