Uranyless Acetone

Uranyless in Acetone for Cryofixation, AFS Application

UranyLess in Acetone is a reagent used for cryo-electron microscopy applications, particularly for freeze substitution techniques (AFS).
This product serves as a safer alternative to traditional uranyl acetate, providing a solution for biological sample preparation while ensuring high-resolution imaging.

Applications: Freeze substitution (AFS), electron microscopy, cryo-applications.
Reference: 11000C-100
Format: 100 ml

MSDS technical file

Protocol AFS : UranyLess Acetone version cryo for AFS application stain sample on bloc

8 hours 2% OsO4 / acetone solution at -90 °C
0.5-hour temperature increase
8 hours 2% OsO4 / acetone solution at -60 °C
0.5-hour temperature increase
8 hours 2% OsO4 / acetone solution at -30 °C
Remove OsO4 solution and add pure acetone glass distilled 100%
1 hour washing acetone glass distilled 100%
Remove acetone and add UranyLess acetone version cryo for AFS application.
8 hours UranyLess acetone version at -30 °C
1.5 hours temperature increase
0 °C End of AFS
As soon as 0 °C was reached, the samples were washed one time with acetone 100% and subsequently embedded in synthetic resin at RT.

With Uranyless Cryo:
membranes are clearly visible

Without Uranyless Cryo :
we didn't distinguish membranes

Compare them

	Aqueous		Acetone	Ethanol
Volume	30ml	200ml	100ml	30ml	200ml
Reference	11000	11000-200	11000C-100	11000E-30	11000E-200
Expiration	12 Months	12 Months	12 Months	12 Months	12 Months
Use	Contrast agent for ultrathin sections or negative staining		Block staining for AFS	Staining of dense and lipid-rich tissues
Use	Contrast agent for ultrathin sections or negative staining		Cryofixation	Staining of dense and lipid-rich tissues
Technique	Manual Staining	Automatic staining for EM stain Leica or RMC grids stainer	Manual Staining	Manual Staining	Automatic staining for EM stain Leica or RMC grids stainer
Container	Airless bottle, no contact with air	HDPE bottle	Glass bottle	Airless bottle, no contact with air	HDPE bottle