Uranyless Ethanol

Uranyless in 30% Ethanol
Ready to Use

UranyLess in 30% ethanol is a reagent specifically developed for tissues that are difficult to contrast, such as very dense, fibrous, or lipid-rich tissues (e.g., cornea). The alcohol acts as a vector, allowing UranyLess to penetrate deep into these tissues and improve contrast.
It serves as an alternative to traditional uranyl acetate for biological sample preparation, providing high-resolution imaging without the use of hazardous materials.
Its ethanol-based formulation enhances contrast penetration while preserving the integrity of lipid structures.

Key Features:

  • Prepared in a 30% ethanol solution in water.
  • Ethanol enhances contrast penetration, particularly in collagen-rich tissues.
  • The 30% ethanol concentration provides an optimal balance for deep tissue penetration while preserving lipid structures.
airless bottle of uranyless in 30% ethanol

Sample Preparation Protocol with UranyLess in 30% Ethanol

1. Contrast the Sample:

  • Apply UranyLess in ethanol to the sample for 1 to 2 minutes.

2. Wash the Sample:

  • Rinse the sample with a drop of 30% alcohol for 1 minute.

3. (Optional) Contrast Enhancement:

  • To further enhance contrast, apply lead citrate (#DM22410) for 1 minute.

Transverse and longitudinal sections of corneal fiber bundles

copyright Dr. Chantal Cazveille-INM Montpellier, France

Compare Them !

AqueousAcetone Ethanol 
Volume 30ml 200ml 100ml 30ml 200ml 
Reference 11000 11000-200 11000C-100 11000E-30 11000E-200 
Expiration12 Months 12 Months12 Months 12 Months 12 Months 
Use Contrast agent for ultrathin sections or negative stainingBlock staining for AFSStaining of dense and lipid-rich tissues
Cryofixation 
Technique Manual StainingAutomatic staining for EM stain Leica or RMC grids stainerManual StainingManual StainingAutomatic staining for EM stain Leica or RMC grids stainer
ContainerAirless bottle, no contact with airHDPE bottleGlass bottleAirless bottle, no contact with airHDPE bottle

SEE ALSO

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Contact

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Mauressac France

+(33) 5 61 73 60 14

info@deltamicroscopies.com

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